4 kilograms). ZERO BIAS - scores, article reviews, protocol conditions and moreNote: For the SpectraMax M2, M2e, M3, M4, M5, M5e, and FlexStation 3. In the M2, M5 and Plus. 2. Serum C-terminal telopeptide of type I collagen (S-CTX), osteocalcin (BGP), and tartrate-resistant acid phosphatase (TRAP) levels in serum and the receptor activator of the NF-κB ligand (RANKL), osteoprotegerin (OPG), and RANK levels in marrow were determinedThe ELISA kits (Lianke Bio, China) were used to detect the concentration of specific cytokines. AM (~0. INTRODUCTION The SpectraMax M2 and SpectraMax M2 e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as well SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. 9000-0133 SpectraMax®MousePad 9000-0972 Packageof5samplemicroplates 2400-2464 DustCover 5000505 CEDeclarationofConformity 5019680 Card,UserGuideDownload The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. In vitro transcription (IVT) is an essential technique for RNA synthesis. After treatment, the Cell Counting Kit-8 (CCK-8) (Dojindo Molecular Technologies, Rockville, MD, USA) was added to the PA and skatole treated hepatocytes and additionally incubated for 1 h. Size and zeta potential of nanoparticles were measured by Zetasizer Nano ZS90 (Malvern Instrument). Products : SpectraMax M3 reader, SpectraMax M4 reader, LMax II (Obsolete), FlexStation 3 reader, SpectraMax L reader, SpectraMax M5/M5e reader How do I know if the LM-1 plate is off or on? The LM1 plate has a push button switch in a cupped-out area on one end. S. While many companies make instruments to detect and measure luciferase activity, identifying the right instrument for you can be difficult because of their high degree of feature variability. alamarBlue reagent volume in a different spheroid microplate formatA SpectraMax M4 microplate reader (Molecular Devices, LLC, Sunnyvale, CA, USA) was used to measure the absorbance at a wavelength of 450 nm. Note: In this user guide, all references to SpectraMax Multi-Mode. SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. 11. Products : SpectraMax M3 reader, SpectraMax M4 reader, SpectraTest Validation Plates LM1, FlexStation 3 reader, SpectraMax L reader, LMax II (Obsolete), SpectraMax M5/M5e reader. 5. The SpectraMax M5e reader offers the additional benefit of being certified . The test conditions are as follows: mobile phase: acetonitrile/H2O (0. SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. 0 Micromoles per Joule, ensuring the highest light output using the least amount of electricity. TSQ was dissolved in Lock’s buffer (pH 7. To quantify F-CHP fluorescence, stained samples were digested in 500 µl of 1 mg/ml proteinase K in water for 3 hours at 60 °C. 2. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies GeneBLAzer® assays. The final concentration of hemoglobin in feces was. Dagda Lab. SpectraMax M4 Multi-Mode Microplate Reader. org) with optimized instrument settings (Table 1) were used to generate and analyze data. 116 SpectraMax M5 and M5e Multi-Mode. Luciferase reporter assays are widely used because of their sensitive detection capacity and broad dynamic range. Description 1. INTRODUCTION The SpectraMax M2 and SpectraMax M2 e Multidetection Readers are monochromator based microplate readers that have 6well to 38 4well microplate reading capability as wellSpectraMax® M4 Microplate Reader Molecular Devices LLC Applications: Absorbance, Cell Health, Cell Viability, Cellular. For SpectraMax L or LMax II, LMax II 384 - no adapter used. 2. 1. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi. If you need to relocate a SpectraMax Multi-Mode Microplate Reader, follow these steps. 4) and cells were stained with 150 nM TSQ for 1 min. Serial number location on Series II and Multi-Mode plate readers. The filtered samples were transferred to a 96-well plate and the fluorescence was measured using a plate reader (Spectramax ™ M4, Multi detection Reader) at excitation and emission wavelengths of λex 339 and λem 390 nm, respectively. Highly selective for dsDNA over ssDNA or RNA. Formazan crystals formed were solubilized in 10 μl DMSO, and absorbance was recorded using a multimode plate reader (SpectraMax M4; Molecular Devices, USA) at 606-nm wavelength. SpectraMax Paradigm SpectraMax i3 SpectraMax Flex-Station 3 SpectraMax F5 SpectraMax M4, M5, and M5e SpectraMax M2, and M2e: Secondary modes: BMG LABTECH: CLARIOstar PHERAstar FS PHERAstar Plus FLUOstar Omega POLARstar Omega PerkinElmer: EnSpire® Multimode Plate Reader EnVision Multilabel Plate. Dumont3, Matthias S. User-installable and removable detection cartridges allow the detector to be configured for specific applications and easily. These settings are also valid for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. Plot the fluorescence intensity versus the cell seeding number. Diclofenac potassium at non-toxic concentration on U87-MG was used as positive control. The Dagda Lab is a mitochondrial biology-oriented research group located at the University of Nevada, Reno School of Medicine that currently spearheads basic and translational research projects supported. We therefore gathered salient. M4 Contracting Ltd, Victoria, British Columbia. Description 1. Luminescence was immediately detected by the SpectraMax M4 Microplate/Cuvette Reader (Molecular Devices, San Jose, CA, USA). The absorbance at 550 nm was recorded using a SpectraMax M4 microplate reader (Molecular Devices) following solvation of formazan with 100 μL DMSO. Like all. Raw data generated on SpectraMax M4 Multi-Mode Microplate Reader is shown in the table. Serum C-reactive protein (CRP) was quantified using the Mouse C-Reactive Protein/CRP Quantikine ELISA Kit. What types of plates can be used with StakMax? SBS Standard 96 and 384 well plates with plate height of 12-16 mm are recommended. 73. To determine the cell viability, thiazolyl blue tetrazolium bromide (MTT) assay was performed. 3. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual- monochromator microplate reader platform offering a wide range of. Cytosolic [K +] was then monitored using a SpectraMax M4 spectrofluorometer (San Jose, CA, USA) by loading AM at 37 ℃ for 40 min with 12 µM APG-2. GSA System Includes: SpectraMax M4 Multi-Mode Spectrophotometer with Tunable Monochromator UV/Vis absorbance Fluorescence intensity, FRET, TRF, TR-FRET and Luminescence as primary modes Tunable. book Page 1 Wednesday, July 7, 2010 2:09 PM. Shop our huge selection of OEM, MRO, construction, industrial, and safety products. SpectraMax® M5. Analytical Technologies Group has deep experience maintaining and repairing Molecular Devices microplate readers, including the SpectraMax® M Series microplate readers (M2, M2e, M3, M5, M5, and M5e). At 48 h after transfection, the Nano-Glo Live Cell Assay reagent was added and the luciferase activity in the medium and cells were measured using a SpectraMax M4 (Molecular Devices, San Jose, CA, USA). Recertification is recommended every two years. This assay kit is optimized for the SpectraMax i3x Multimode Microplate Reader with a preconfigured protocol in SoftMax Pro Software for rapid. 22 μM was mixed with 10-μl enzyme giving a final enzyme concentration in the. book Page 1 Wednesday, July 7, 2010 2:09 PM M4 SpectraMax M5 SpectraMax M5e Absorbance FluorescenceIntensity Luminescence Time-Resolved Fluorescence FluorescencePolarization ReadModes The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. . 3. SpectraMax Gemini XPS/EM Register or Sign In. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. The microplate was exposed to an excitation wavelength of 530 nm (peak excitation is 540 nm), and the emission at 590 nm (peak emission is 580 nm) is recorded. The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and drug discovery screening. Cell viability (%) was calculated as the ratio of the number of living cells in treated samples to that of the control [15,30]. FlexStation ®, SpectraMax. 4. Table 2. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) in 96- and 384-well plates. 5. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. Transfected cells were seeded into 6-well plates at a density of 200 cells/well. 05, ** p <. Raw data generated on SpectraMax M4 Multi-Mode Microplate Reader is shown in the table. 1. . C. Then 10 µL of CCK-8 reagent was added to each well, and cell growth was measured at 490 nm using the SpectraMax M4 Multimode Microplate reader (Molecular Devices, San Jose, CA, USA). Images were visualized using a fluorescence microscope (Nikon, Japan) . Products : SpectraMax M3 reader, SpectraMax M4 reader, SpectraTest Validation Plates LM1 , FlexStation 3. The SpectraMax Vertical 1000 uses only the highest-efficacy ams-Osram LEDs with efficiencies up to 4. Molecular Devices의 Microplate Reader 및 washer와 원활하게 호환되는 강력한 벤치탑 Microplate stacker입니다. SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. Table 2. Data was calculated subtracting OD440 recorded with the final OD600 values. ). Rhizosphere soil microbial communities substantially impact plant growth by regulating the nutrient cycle. The SpectraMax®M3, M4, M5, and M5eMicroplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port. 5. SpectraMax M2 and M2e Performance Specifications SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers User Guide — 0112-0102 Rev. For SpectraMax L or LMax II, LMax II. e. : 915 515 403 • Fax: 914 334 545 • e-mail: info@bionova. Molecular Devices SpectraMax ® M3/M4/M5/M5e Multi-Mode Microplate Readers + + Molecular Devices SpectraMax. After incubation, the fluorescence intensity was measured using Spectramax M4 (Molecular Devices) (excitation: 380 nm emission: 460 nm). Following incubation, the media was aspirated completely and MTT formazan crystals formed were dissolved by the addition of dimethylsulfoxide, and the reading was taken at 570 nm using SpectraMax M4. Find M4 Group Marketing Specialists in Victoria, with phone, website, address, opening hours and contact info. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi-mode. Immunodetection Protocols for Cell Culture. spr SpectraMax M2 M2e M3 M4 M5 M5e SpectraTest ABS2 v1. Measurements of temperature increase (ΔT) during the exposure of MNP/AMF hyperthermiaThe culture medium was removed, and the blue product was dissolved in DMSO (100 μl). Because many reagents can be fluorescently labeled, spectrofluorometers are used to detect many biological and chemical processes. One of the. • Tel. Titration of human serum and plasma (heparin) within the working range of the assay. Images were taken using a fluorescence microscope (Nikon 80i, Japan). Flasks containing 100 mL of YM broth were inoculated with 100. SpectraMax M3, M4, M5, and M5 e. 6: bioNova científica, s. C. 1 M Tris solution and the fluorescence of the solution was measured using a fluorometer (SpectraMax M4, Molecular Devices). 4) In vivo pharmacokinetic studies. SpectraMax iD5; SpectraMax Mini; SpectraMax Mini Install Guide; SpectraMax Mini Reader Plug-In Download Page (required for SoftMax Pro 7. 2. Choose from a three- (M3), four- (M4), or Cables. 1. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). Spectrofluorometers or plate readers with fluorescence detection typically offer greater sensitivity and a wider dynamic range compared to absorbance detection. Large Molecule Bioanalysis. (Fair Lawn, NJ, USA). The OD value of each well was determined using a SpectraMax M4 Multimode Microplate reader (Molecular Devices, USA), and the concentration of cytokines was calculated by comparing their OD value with the corresponding standard curve. The medium was then removed and 100 μl of DMSO was added to dissolve the formazan crystal. ATP concentration (M) Average RLU SpectraMax L SpectraMax i3x (cartridge) SpectraMax Mini SpectraMax iD5 SpectraMax i3x (onboard) SpectraMax M5eThe SpectraMax® Paradigm® Multi-Mode Microplate Reader measures absorbance, fluorescence, time-resolved fluorescence (including HTRF), fluorescence polarization, AlphaScreen®, AlphaLISA®, and luminescence assays for up to 1536-well plates. Molecular Devices SpectraMax ® M3/M4/M5/M5e Multi-Mode Microplate Readers + + Molecular Devices SpectraMax. spr SpectraMax M2 M2e M3 M4 M5 M5e SpectraTest ABS2 v2. The percentage of viability of each treatment group in different. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. Steady-state kinetics measurements were performed at 25°C in 96-well plates (Corning) using a SpectraMax M4 spectrophotometer (Molecular Devices) in PBS buffer. Other technologies such as imaging. Molecular Devices recommends that you use high-quality, doubleshielded cables to connect your SpectraMax. SpectraMax i3(x) SpectaTest ABS1 Location: Reader Validation-Plate Abs A reminder about recertification was added. With optimized reagents, option to operate the. Bioz Stars score: 98/100, based on 1 PubMed citations. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi. SpectraMax M3 Multi-Mode Microplate Reader with 1-Year Service Contract. , USA). Background-subtracted data values (mean +/- SD) are graphed. 1 Figure from 528 Publications Molecular Devices LLC. The whole process was protected from direct light. Absorbance was read at 570 nm with SpectraMax M4 microplate spectrophotometer (Molecular Devices, CA). Table of Contents. Assessment of Neurite Outgrowth. 1. Instruments Ltd. Ninety microliters of 2-chloro-4-nitrophenyl-α- d -maltotrioside substrate dilutions from 5000 to 1. 75 × 10 5) suspended in PBS in 96-well dark plates incubated with NP or SiO 2 for 1 h at 37 °C. Multi-Mode Microplate Readers. The samples were centrifuged to pellet the remaining intact erythrocytes, and supernatants were collected. Then, the fluorescence intensity was quantified by using a spectrophotometer (Spectramax M4, MolecularDevices, San Jose, CA, USA) at λ ex: 579 nm and λ em: 599 nm. Luciferase reporter assays are widely used because of their sensitive detection capacity and broad dynamic range. Other technologies such as imaging. Products : SpectraMax M3 reader, SpectraMax M4 reader, SpectraTest Validation Plates FL1, Gemini EM reader, Gemini XPS reader, SpectraMax M5/M5e reader My SpectraTest FL one plate is failing for LLD for the wet test. The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port. ATP standard curves read on SpectraMax microplate readers. For a complete list of the instruments supported by this release of the SoftMax. N2a cells were induced for neurite growth by adding nerve growth factor (NGF) (50 ng/ml). 2. 2. After incubation, the biofilms were washed with PBS until fully remove crystal violet solutions and then, solubilized by adding a 150 μL acetic acid. Phillips5, Martin A. The adherent cells were incubated in 100 μl of lysis buffer on ice for 20 min. MEK Inhibition Induces Therapeutic Iodine Uptake in a Murine Model of Anaplastic Thyroid Cancer Oussama ElMokh1, Vincent Taelman2, Piotr Radojewski2, Matthias A. The SpectraMax M2/M2e User Guide is a comprehensive manual that provides detailed instructions on how to operate, maintain, and troubleshoot the SpectraMax M2 and M2e microplate readers. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. 12. SpectraMax M Series Multi-Mode Microplate Readers | Molecular Devices · 2020-04-14 · use two scanning monochromators so the user can determine optimal excitation and emission settings, of 8 /8 Match case Limit results 1 per pageThe culture medium was removed, and the blue product was dissolved in DMSO (100 μl). The lower critical solution temperature (LCST) at which phase transition occurs was measured via plate reader absorbance for n = 3 samples (SpectraMax M4; Molecular Devices). The reaction systems were cultured in 37°C for 1 h, and the OD405 values were read on SpectraMax M4 (Molecular devices). Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader customized to your specific applications or budgetary needs, while optional. Data are from ≥5 independent experiments. Louis, MO, USA). The SpectraMax M2 and M2e are versatile and reliable. 7. To assess the effect of CAD drugs on lysosomal activity in BMdM, fluorescence analysis using the DQ Green BSA probe was performed. Choose from a three- (M3), four- (M4), or Qualify the luminescence performance of the SpectraMax . Learn how to easily set up an efficient and informative plate read by using SoftMax Pro 6 Microplate Data Acquisition and Analysis software. When using a SpectraMax M3 Reader, the GxP audit trail incorrectly reported the instrument as a SpectraMax M4 Read er. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. 2 only) SpectraMax M-series Microplate readers. Our extensive SpectraMax® line of user-friendly microplate readers – the industry’s most cited brand – provide great flexibility for labs conducting applications ranging from. The SpectraMax M4 reader had identical performance specifications to the SpectraMax M5 reader for absorbance and fluorescence detection. * Nunc. spectramax multi-mode microplate Readers The SpectraMax® M3, M4, M5 and M5 Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. Choose from a three- (M3), four- (M4), or We would like to show you a description here but the site won’t allow us. Then the formazan crystals were solubilized in dimethyl sulfoxide, and the absorbance was recorded at 572 nm wavelength using a multimode plate reader (SpectraMax M4, Molecular Devices, USA). Colony formation assays were performed to measure cell proliferation. 306 plate air cal fail,low light. For more detailed information and technical support of Life Technologies assays, please call 1-800-955-6288 and enter extension 40266 or email drugdiscoverytech@lifetech. 7. TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-Mode. Table 2. The Molecular Devices SpectraMax® M5/M5e Microplate Reader was tested for compatibility with Life Technologies Omnia® Assays. WARNING! The instrument weighs approximately 36 pounds (16. ® reader to the computer. The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate reading capability. When the user applies a set temperature of 37°C, the top is heated to 37. Dual monochromators for assay flexibility With SpectraMax M Series readers, there is no need to utilize expensive filters to optimize detection levels and background. M2 M2e M3 M4 M5 M5e ABS1 Location: Reader Validation-Plate Abs A reminder about recertification was added. SpectraMaxi3,M3,M4,M5,M5e,andFlexStation 3instruments G03 through G08forSpectraMaxLinstrument Cross-talk D09 through D11,E09 through E11,andF09 through F11 for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). Published Dec 16, 2020 | Updated Dec 16, 2020. Optimized for fluorescence microplate readers. All of the experiments were performed in triplicate, and the results were represented as mean. Products : SpectraMax M2/M2e reader, SpectraMax M4 reader, SpectraMax M5/M5e reader, Gemini XPS reader, SpectraMax 190 reader, VersaMax reader, Gemini EM reader, SpectraMax Plus 384 reader. Hexokinase reagent (Eagle Diagnostics) was added and the reaction absorbance was read at 340 nm using a SpectraMax M4 multimode plate reader (Molecular Devices). Raw data generated on SpectraMax M4 Multi-Mode Microplate Reader is shown in the table. Molecular Devices LLC spectramax m4 multimode microplate reader page 12 25 Spectramax M4 Multimode Microplate Reader Page 12 25, supplied by Molecular Devices LLC, used in various techniques. alamarBlue reagent volume in a different spheroid microplate formatAfter incubating for 10 min at room temperature, the absorbances of standards and samples were measured at 450 and 590 nm, 12 using a Spectramax M4 spectrophotometer (Molecular Devices). . Place the instrument on a level surface, away from direct sunlight, dust, drafts, vibration, and moisture. normalized response (Graphpad Prism 8. When a feature or capability applies to only certain readers, this exception is noted. 005) Photometric PrecisionThe SpectraMax Paradigm Multi-Mode Detection Platform is a modular multi-mode microplate detector. Data are presented as mean values ± standard. SpectraMax M4 Multi-Mode Microplate Reader. The enzymatic reaction was triggered by addition of luminol and the chemiluminescence signal was monitored at 37 °C using a microplate reader SpectraMax M4 (Molecular Devices, Sunnyvale, CA, USA), with one acquisition every 2 s for 300 min. Molecular Devices SpectraMax M5 User Manual (86 pages) Multi-Mode Microplate Readers. Molecular Devices’ unique SpectraDrop™ MicroVolume Microplate offers the highest throughput solution for low volume measurement available on the market today. SpectraMax® iD5. Molecular Devices SpectraMax M4 upgradable microplate/cuvette reader (90V to 240V) with current version of SoftMax Pro. The medium was then removed and 100 μL of DMSO was added to dissolve the formazan crystal. trophotometer (SpectraMax M4, Molecular Devices, USA). StakMax Handling Stacker Base Unit. The SpectraMax M4 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96 and 384-well microplates. Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader. Choose from a three- (M3), four- (M4), orThe absorbance of the biofilm was measured at 595 nm using a microplate reader (SpectraMax M4) and the results were expressed as percentage changes with respect to the control (without compounds). The fluorescence intensity was measured as ratio of values at an excitation at 340 nm and 380 nm with an emission at 510 nm, using Spectramax M4 (Molecular Devices). 316 grating motor driver faulty. Walter2, and Roch-Philippe Charles1 1Institute for Biochemistry and Molecular. 2 only). ELISA standard curves were generated using a four-parameter logistic curve model (GraphPad Prism 8, GraphPad Software; San Diego, CA, USA). Qualify the luminescence performance of the SpectraMax . The IP-10 standard curve was prepared as described in Section 10. Calpain activity assay The cells were treated with different drugs for 24 hours and were resuspended in 100 μL extraction buffer and homogenized by pipetting. The IL-10 standard curve was prepared as described in Section 10. The available phosphorus in the soil was leached by double-acid extraction (0. The 96-well plates were read on the SpectraMax M4 plate reader at the following wavelengths: green fluorescence at 488 ex. Microplate Readers include the M3, M4, exception is noted. Quantitative Reverse Transcription Polymerase Chain Reaction (qRT-PCR) Total RNA was extracted at 48 h after exposure to volasertib, genistein, or gefitinib and quantified by a NanoDrop spectrophotometer (Thermo Scientific; Wilmington, DE, USA). The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi. FilterMax F3 and F5 Multi-Mode; SpectraMax Paradigm® Multi-Mode. Standard features include a cuvette port,. Note: In this user guide, all references to SpectraMax Multi-Mode. The absorbance change at 620 nm was measured by a SpectraMax M2 Microplate Reader (Molecular Devices). 025 mol/L sulfuric acid) and then measured following the molybdenum antimony colorimetric method (multifunctional microplate reader, SpectraMax M4, MD company, USA). SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 This document is provided to customers who have purchased Molecular Devices,…spectrophotometer (SpectraMax M4, Molecular Devices, San Jose, CA, USA) with excitation λ 485nm and emissionλ 535nm. Sandwich ELISA - Human TNF alpha ELISA Kit, Fluorescent (ab229399) To learn more about the advantages of recombinant antibodies see here. , and red fluorescence at 543 ex. Results. Italic and boldface type are used for emphasis. . The absorption value at 570 nm was measured using a Molecular Devices SpectraMax M4 plate reader, and the absorbance at 690 nm was subtracted as the background. 8. Record fluorescence emission data in kinetic mode for 2 min with 20 s between reads to ensure that interaction has reached equilibrium and take average. At the end of the incubation period, MTT was added and the plates were incubated for 4 h at 37°C. Control AM were incubated in the same buffer for 1 h at 37 °C. The absorbance of the biofilm was measured at 595 nm using a microplate reader (SpectraMax M4) and the results were expressed as percentage changes with respect to the control (without compounds). The SpectraTest® Validation Plates are valuable tools for verifying that absorbance-, fluorescence-, and luminescence-capable microplate readers are operating correctly for GMP and GLP laboratories. 05 mol/L hydrochloric acid and 0. Living cells were then incubated with 100 µl of medium assay provided by the assay kit. AquaMax® 4000. . SpectraMax® M3, M4, M5, and M5e Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010 M3-M5e_manual. 315 can't find zero order. 90-0083 series): Excitation 530 nm,. . 37. Description 1. We therefore gathered salient. Dumont3, Matthias S. TheSpectraMax®M3Multi-ModeMicroplateReader,SpectraMax®M4Multi-ModeMicroplate Reader,SpectraMax®M5Multi-ModeMicroplateReader,andSpectraMax®M5eMulti-Mode MicroplateReaderaredualmonochromator,multi-detectioninstrumentswithatriple-mode cuvetteportanda6-wellto384-wellplatereadcapability. 3, La Jolla, CA, USA). Tris solution and the fluorescence of the solution was measured using a fluorometer (SpectraMax M4, Molecular Devices). The aim of the present study was to investigate the preventive effects of. Background-subtracted data values (mean +/- SD) are graphed. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi-mode. 0 compensates for most changes in environmental temperature or pressure. The following document is intended to demonstrate setup of this instrument. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. 4. SpectraMax 190: SpectraMax 340PC384: SpectraMax Plus384: VERSAmax: Vmax: Tecan: Sunrise: Trinean: DropSense96: AMBIENT TEMPERATURE STORAGE: Liconic: LPX 220 Hotel: LPX 440 Hotel: Thermo Fisher Scientific: Cytomat 10 Hotel: Cytomat 2 Hotel: Cytomat 24 Hotel: Cytomat 8 Linear Hotel: Cytomat 9 Hotel (MPH) BAR CODE. Standard features include a cuvette port, spectral scanning in 1 nm increments, and up to six wavelengths per read. The optical absorbance of the plate at 450 nm was measured using a multifunctional microplate reader SpectraMax M4 (Molecular Devices, LLC, San Jose, CA, USA). +1 250-415-0785. Western blot apparatus (BIO-RAD Laboratories). Cells were stained with dichlorofluorescein diacetate, fixed, and immediately analyzed using a multianalytic validation system (SpectraMax M4). Specifications LUMINESCENCE PHOTOMETRIC PERFORMANCE Detection limit (384-well microplate) 10 amol/well alkaline phosphatase, 200 µL Wavelength range 250–850 nm TIME-RESOLVED FLUORESCENCE PERFORMANCE SpectraMax M3 reader SpectraMax M4 reader SpectraMax M5 reader SpectraMax M5e reader Detection modes Absorbance ü ü ü ü Fluorescence intensity ü ü ü ü Luminescence ü ü ü ü Time-resolved fluorescence ü ü ü Fluorescence polarization ü ü HTRF ü Upgrade options TRF, HTRF, FP HTRF, FP HTRF N/A Plate formats The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. The polysaccharide HPA with different concentrations (0, 25, 50, and 100 μg/mL) was added and incubated with cells for 24 h. For the FlexStation 3 Reader - Keep the Black Plate Adapter in place fro both Top and Bottom reads. The user must ensure that the meniscus is comfortably above the light beam in standard cuvettes and that the sample chamber in a microcuvette is aligned properly with the beam. Here, we present an approach to predict UV-visible absorption spectra of solvated aromatic molecules by quantum chemistry (QC) and machine learning (ML). The drawing shows a standard microplate of 15 mm height covered with a lid. Full content visible, double tap to read brief content. Description 1. D 1 1. Technical specifications Detection limit for ATP Glow 0200-7012 (LUM) Optimized Guaranteed* 96-well (200 µL) 1. Background-subtracted data values (mean +/- SD) are graphed. Choose from a three- (M3), four- (M4), or five- (M5/M5e) mode reader customized to your specific applications or budgetary needs, while optional capabilities allow you to upgrade with other detection modes at a later time. While many companies make instruments to detect and measure luciferase activity, identifying the right instrument for you can be difficult because of their high degree of feature. The SpectraMax® M3 Multi-Mode Microplate Reader dual monochromatic optics allows the widest range of applications to be utilized for bioresearch applications all without the need to change filters, and provides three modes of detection in one platform. FlexStation™ III. • SpectraMax® M3/M4/M5/M5e Multi-Mode Microplate Readers • SpectraMax® Paradigm® Multi-Mode Microplate ReaderThe medium was replaced with CM from the macrophages of the different groups and then incubated for 48 or 72 h. Click Read. Calpain activity assay The cells were treated with different drugs for 24 hours and were resuspended in 100 μL extraction buffer and homogenized by pipetting. Absorbance was measured at 570 nm using a multimode reader (Spectramax M4, USA) (Kalvala et al. The content was vortexed for 1 min and incubated in the dark for 20 min at 25°C. This platform allows the. After incubation at 37 °C for 3 h, the fluorometric detection of cleaved AFC and chromogenic product of pNA was performed on a Multimode Plate Reader (Spectramax M4, Molecular devices, USA) using a 400 nm excitation filter and a 530 nm emission filter for detection of caspase 3 and 410 nm wavelength for detection of caspase 9 using. Description 1. The SpectraMax M4 reader had identical performance specifications to the SpectraMax M5 reader for absorbance and fluorescence detection. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. Fluorescence Microplate Reader Comparison Chart. SpectraMax® M4 Multi-Mode Microplate Reader MLDVM4-Dual monochromatic optics -Wide range of applications -Top or bottom read modes The SpectraMax M3 Multi-Mode Microplate Reader provides three modes of detection in one platform The SpectraMax M3 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range. SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. SpectraMax iD5; SpectraMax Mini; SpectraMax Mini Install Guide; SpectraMax Mini Reader Plug-In Download Page (required for SoftMax Pro 7. Keywords: spectramax m2 systems, spectramax m2e systems, spectramax m2/m2e readers, pathcheck sensor, spectratest abs1 absorbance, stakmax stacker Created Date:. Like all SpectraMax® Readers, the new M3 and M4 readers leverage patented technologies to deliver added value to users. TYPICAL SAMPLE VALUES Sensitivity: The calculated minimal detectable dose (MDD) is 15. for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. Luciferase reporter assays are widely used because of their sensitive detection capacity and broad dynamic range. 9000-0756. In the competitive field of drug development where timely go/no-go decisions must be made to bring effective class-leading drugs to market, it is vital to work with a CRO that can provide highly. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. 116 SpectraMax M5 and M5e Multi-Mode. Lower limits of detection for ATP determined using the ATPlite . Table of Contents. The instrument reads all Plate sections in the experiment. Subsequently, 20 µl CCK-8 solution (5 mg/ml) diluted in PBS was added to each well for another 1 h and the absorbance was measured at 450 nm using a SpectraMax M4 microplate reader (Molecular Devices, LLC). , cyan fluorescence at 458 ex. 1 Measurement of Intestinal Permeability (In Vivo). In luminescence mode, if you are specifying a wavelength for the read, you can select an emission bandwidth of either 15 nm or 25 nm. In. Examples: “Press carefully to engage,” “ Do not press down. > spectramax m4 microplate reader. SpectraMax® L (software version 5. The ELISA kits (Lianke Bio, China) were used to detect the concentration of specific cytokines. To avoid injury, it is recommended that two. The viability of cells was determined by using SpectraMax® M4 Multi-Mode Microplate Reader (Molecular Devices, Sunnyvale, CA, USA) at 570 nm absorbance . The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. The fluorescent signal was measured by multiplate reader (Spectramax M4, Molecular Devices LLC, Sunnyvale, California, USA). Add To My List; Create New List; Quickview. SPECTRAmax 340PC Microplate Spectrophotometer Operator’s Manual 1-3 Introduction General Overview The SPECTRAmax ® 340PC incorporates a holographic grating. Next to the pushbutton is a green LED. The SpectraMax® M3, M4, M5 and M5e readers offer a modular, upgradeable dual monochromator microplate reader platform with a wide range of high performance multi. 4) and cells were stained with 150 nM TSQ for 1 min. Ninety microliters of 2-chloro-4-nitrophenyl-α- d -maltotrioside substrate dilutions from 5000 to 1. 313 reference gain check fail. After 7 days of culture, the colonies generated. FlexStation™ I. For more detailed information and technical support of Molecular Devices instruments or software, Luciferase Detection Instrumentation Guide. SpectraMax® Paradigm® Multi-Mode Detection Platform. MTT was then removed, and the formed formazan crystals were then dissolved in 150‐μl DMSO. "With the launch of the Molecular Devices® SpectraMax® M3 and M4 Multi-Mode Microplate Readers, we now offer a full range of multi-mode detection capabilities within our SpectraMax® product line," said Andy Boorn, President of MDS Analytical Technologies. S. Description 1. Colony Formation Assay. 1. Also, when using a SpectraMax M4 Reader, the GxP audit trail incorrectly re ported the instrument as a SpectraMax M3 Reader. Our absorbance plate readers feature our PathCheck Sensor. A series of Toll-like receptor 7 (TLR7)-specific antagonists and extensive structural analysis reveal the open conformation of the receptor and the structural basis of TLR7 antagonism. spr SpectraMax Plus 384. 01) + 0. es • Flexibility for your assay needs: Robotics compatibility for increased throughput:すべてのSpectraMax® Mシリーズマルチモードマイクロプレートリーダーで、マイクロプレートおよびキュベットを使用して、紫外光・可視光吸光度ならびに蛍光強度を測定できます。.